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Fig. 5 In vitro B lymphopoiesis of HSCs, LMPPs, CLP in the 1%BSA X-VIVO15 complete medium. (A)The development of B-lineage cells was analyzed via flow cytometry with antibodies against CD11b and CD19 during cultured for 7 d. (B) The increase in the number of CD19 + and CD11b + cells under culture. The percentage mean each cell accounted for the total cell frequency. **P<0.01. ***P<0.001. ****P<0.0001

Journal: BMC immunology

Article Title: In vitro differentiation of common lymphoid progenitor cells into B cell using stromal cell free culture system.

doi: 10.1186/s12865-025-00694-8

Figure Lengend Snippet: Fig. 5 In vitro B lymphopoiesis of HSCs, LMPPs, CLP in the 1%BSA X-VIVO15 complete medium. (A)The development of B-lineage cells was analyzed via flow cytometry with antibodies against CD11b and CD19 during cultured for 7 d. (B) The increase in the number of CD19 + and CD11b + cells under culture. The percentage mean each cell accounted for the total cell frequency. **P<0.01. ***P<0.001. ****P<0.0001

Article Snippet: Chemicals, antibodies and analytical flow cytometry Recombinant mouse stem cell factor (SCF), mouse Flt3 ligand (Flt3L) and mouse IL-7 were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: In Vitro, Flow Cytometry, Cell Culture